Chine (Rép. pop) Pro / B2B
Pro / B2B

Chromatography System

Hanbon advanced chromatography systems has reliable performance and flexible configuration, and can provide Bio-Lab® laboratory chromatography system, Bio-Pro® pilot&process chromatography system, etc.

ommon Principles Of Process Scale-Up: Linear Scale-Up

Linear scale-up is the most common principle used in process scale-up. By linear scale-up, we mean that we can obtain almost uniform bioprocess chromatography results from laboratory scale to pilot results or industrial production level. The core idea is to keep the column height and linear flow rate constant and only scale up the column diameter and volume flow rate. A fixed column height and linear flow rate theoretically ensures the same retention time, the number of CVs used in chromatography and the number of CVs of the eluted sample, maximizing the same purification results. Theoretically, this linear scale-up of the purification separation behavior should be very similar, thus ensuring a successful scale-up of the ion chromatography process. However, some additional factors need to be considered, including a decrease in column tube support (increased pressure drop), column efficiency/column bed instability, sample diffusion (peak spreading), and dead volume of the system and tubing.

Conversion Of Linear Flow Rates (Cm/H) To Volumetric (Ml/Min) Flow Rates

When comparing results from columns of different sizes, it is convenient to express the flow rate as a linear flow rate (cm/h). However, flow rates are often measured as volumetric flow rates (ml/min). In order to convert between linear and volumetric flow rates, one of the following equations can be used .

Linear flow rate (cm/h) to volumetric (ml/min) flow rate

Volumetric flow rate (ml/min) = linear flow rate (cm/h) / 60 x column cross-sectional area (cm2)

=Y/60×π×d2/4

Y = linear flow rate (cm/h)

d = internal diameter of the column (cm)

e.g.:

There is a glass column (inner diameter 1.6 cm), when the linear flow rate is 150 cm/h, what is the volumetric flow rate?

Y=Linear flow rate = 150cm/h

d = inner diameter of the column = 1.6 cm

Volumetric flow rate = 150 x π x 1.6 x 1.6/(60 x 4)

From volumetric flow rate (ml/min) to linear flow rate (cm/h)

Linear flow rate (cm/h) = volumetric flow rate (ml/min) x 60/column cross-sectional area (cm2)

=Z×60×4/π×d2

Z = volumetric flow rate of the column (ml/min)

d = inner diameter of the column (cm)

e.g.:

There is a glass column (inner diameter 0.5 cm), when the volumetric flow rate is 1 ml/min, what is the linear flow rate?

Z = volumetric flow rate of the column = 1 ml/min

d = inner diameter of the column = 0.5 cm

Linear flow rate = 1 x 60 x 4/(π x 0.5 x 0.5) cm/h = 305.6 cm/h

We provide chromatography step by step method, chromatography purification method, chromatography process example, anion exchange chromatography elution, equipment used for chromatography, etc. For more information, please feel free to contact us!

Localisation : No.1-9 jixian road, economic development zone, 223005 Jiangsu,
Personne à contacter : com hanbon, 86 0517 83706900

Bonne affaire : acheter au vendeur

Consultez notre guide pour effectuer une transaction en toute sécurité ! En aucun cas Algomtl ne peut être tenu responsable des annonces publiées. Seule la responsabilité du vendeur ou de l'acheteur en cas de litige est engagée.
Nous vous invitons à lire nos conditions générales d'utilisations. Vous pouvez aussi vous rendre sur nos FAQ et consulter notre page d'informations sur les risques liés à la contrefaçon.
Cette page concerne les importateurs et exportateurs de
Chromatography System

Rechercher dans la catégorie : Pro / B2B
Rechercher dans la catégorie :
system, chromatography
France 13008 Téléphonie
IPHONE 3G 3GS GRADE B/C 35E HT

Lundi 27 avril 2015

Quantité : 230 - Prix : 35HT

- IPHONE 3G 3GS GRADE B/C 35E HT - Quantité : 225 Prix : 35HT BONJOUR A LA VENTE UN LOT DE 225 IPHONE 3G 3GS 8GIGA 16GIGA 32GIGA GRADE B/C FONCTIONNEL TARIF 35EURO HT.LIVRAISON PARIS OU MARSEILLE OFFERTE. NOUS AVONS UN AUTRE LOT DE NOKIA ASHA A25 EURO HT. MINIMUM DE COMMANDE 25PIECES...

WLF

  • 13008 - marseille
  • +33 6 98 19 24 26
Automatic Gear Pump Liquid Filling Machine FP-465

Jeudi 03 avril 2025

Automatic Gear Pump Liquid Filling Machine FP-465 is designed and engineered to produce outstanding quality and precision filling suitable for various products like shampoo, hot sauce, lubricant oil etc. FP-465 can also replace piston or peristaltic module for the flexibility of filling demands...

Autopack Co.,Ltd

Benchtop Pump Dispenser Capper CW-100

Jeudi 10 avril 2025

Benchtop Pump Dispenser Capper CW-100 is ideal capper for any kind of screw cap. Pump dispenser cap compatible. FEATURES: - Capping torque can be easily adjusted and accurately set - Pump Dispenser Cap compatible - Adjust vertically by only one handle - Benchtop model breaks space...

Autopack Co.,Ltd